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OPTOBIOTICS: Novel antibacterial proteins activated by blue light

Country of Origin: Spain
Reference Number: TOES20190506004
Publication Date: 6 May 2019

Summary

A Spanish public research institution has developed a chimeric protein that, when expressed in bacteria, changes its structure upon absorption of blue light, thus generating protein aggregates (amyloids) that inhibit bacterial growth

Industrial partners from the pharmaceutical industry are being sought to collaborate through a patent licence agreement

Description

In this patent, the power of optogenetics to engineer proteins as light-responsive switches has been used to control the balance between solubility and amyloid aggregation for LOV2-WH1, a chimera between the plant blue light-responsive domain LOV2 and the bacterial prion-like protein RepA-WH1.
In the darkness and in vitro, LOV2-WH1 nucleates the irreversible assembly of amyloid fibres into a hydrogel. However, under blue light illumination LOV2-WH1 assembles as soluble oligomers.
When expressed in the bacterium Escherichia coli, LOV2-WH1 forms in the darkness large intracellular amyloid inclusions compatible with bacterial proliferation. Strikingly, under blue light LOV2-WH1 aggregates decrease in size while they become detrimental for bacterial growth.
LOV2-WH1 optogenetics governs the assembly of mutually exclusive inert amyloid fibres or cytotoxic oligomers, thus enabling the navigation of the conformational landscape of protein amyloidogenesis to generate a new kind of photo-activated anti-bacterial devices (optobiotics).

Advantages and Innovations

A synthetic protein construction (LOV2-WH1) is activated as an anti-microbial by a physical, harmless stimulus: blue light

Blue light absorption by LOV2-WH1 stimulates the assembly of anti-bacterial protein particles whereas, in the darkness, these are inert

Mobilization of LOV2-WH1 through vectors/bacteriophages would enable its usage against a broad spectrum of bacterial pathogens

LOV2-WH1 is amenable to photo-therapy procedures aiming to combat bacterial skin infections, or to de-contaminate pathogens from surfaces

In mixed bacterial populations assembled in an industrial bioprocess, optogenetic activation of LOV2-WH1 can be used to selectively eliminate a subpopulation, e.g. once achieved its intended task

Stage Of Development

Available for demonstration

Requested partner

Industrial partners from the pharmaceutical industry are being sought to collaborate through a patent licence agreement

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